Journal: Clinical and Experimental Immunology

Article Title: Modulation of the immune response to Mycobacterium tuberculosis during malaria/ M. tuberculosis co‐infection

doi: 10.1111/cei.12861

Figure Lengend Snippet: CD4+ T cell counts from patient groups. Patients were grouped according to disease diagnosis plus or minus tuberculosis (TB) chemotherapy into the following groups: controls (Cont), latent TB (Lat‐TB), active TB without chemotherapy (TB+drug–) or with chemotherapy (TB+drug+), TB‐HIV co‐infection (TB+HIV+) and TB‐malaria (TB+MP+) co‐infection. CD4 counts were determined using blood collected in ethylenediamine tetraacetic acid (EDTA) analysed with an automated Cyflow SL.3 cell cytometer. Counts are expressed as cells/μl. Graph shows median and 5–95 percentiles. Statistical significance between groups was determined using a Kruskal–Wallis test with Dunn's multiple comparison correction; *P < 0·05; **P < 0·01; ***P < 0·001; ****P < 0·0001. Significant differences among controls and latent TB and active TB groups are shown in grey. Significant differences among active TB groups are shown in black.

Article Snippet: Blood samples were collected in ethylenediamine tetraacetic acid (EDTA) vacutainer blood collection tubes (BD Biosciences, San Jose, CA, USA) and CD4 + T cell counts were measured within 5 h of sample collection using a Cyflow SL.3 cytometer for automated CD4 + counts, as per the manufacturer's instructions (Partec, Nuremberg, Germany).

Techniques: Infection, Cytometry